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α-1,3-N-acetyl-galactosaminyltransferase; BgtA

α-1,3-N-acetyl-galactosaminyltransferase; BgtA

E. coli recombinant α-1,3-N-acetyl-galactosaminyltransferase from Helicobacter mustelae

E.C. No.:     2.4.1.40
CAzy:          GT6
MW:            37 kDa
Purity:          min 95% by SDS-PAGE

Unit Define:
One unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol of  GalNAcα1,3(Fucα1,2)Gal from Fucα1,2Gal per minute at 37 °C.

Reaction catalysed:
UDP-N-acetyl-alpha-beta-D-galactosamine glycoprotein-alpha-L-fucosyl-(1->2)-D-galactose <=> UDP glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1->3)-(alpha-L-fucosyl-(1->2))-beta-D-galactose

  • Acts on blood group substance H
  • Can use a number of 2-fucosyl-galactosides as acceptors

References:
J Am Chem Soc, 2008, 130(44), 14420-14421.

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SKU: EN01004 Categories: , ,

Description

glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; A-transferase; histo-blood group A glycosyltransferase (Fucalpha1->2Galalpha1->3-N-acetylgalactosaminyltransferase); UDP-GalNAc:Fucalpha1->2Galalpha1->3-N-acetylgalactosaminyltransferase; alpha-3-N-acetylgalactosaminyltransferase; blood-group substance alpha-acetyltransferase; blood-group substance A-dependent acetylgalactosaminyltransferase; fucosylgalactose acetylgalactosaminyltransferase; histo-blood group A acetylgalactosaminyltransferase; histo-blood group A transferase; UDP-N-acetyl-D-galactosamine:alpha-L-fucosyl-1,2-D-galactose 3-N-acetyl-D-galactosaminyltransferase; UDP-N-acetyl-D-galactosamine:glycoprotein-alpha-L-fucosyl-(1,2)-D-galactose 3-N-acetyl-D-galactosaminyltransferase; BgtA

Additional References:

Kobata A, Grollman EF, Ginsburg V.    An enzymic basis for blood type A in humans.    Arch. Biochem. Biophys. 124 (1968) 609-12.    Takeya A, Hosomi O, Ishiura M.    Complete purification and characterization of alpha-3-N-acetylgalactosaminyltransferase encoded by the human blood group A gene.    J. Biochem. (Tokyo). 107 (1990) 360-8.    Yates AD, Feeney J, Donald AS, Watkins WM. Characterisation of a blood-group A-active tetrasaccharide synthesised by a blood-group B gene-specified glycosyltransferase.    Carbohydr. Res. 130 (1984) 251-60.

Additional information

Package

1 Units, 5 Units, 25 Units

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