Glycan Arrays
Glycan arrays provide a convenient tool to determine the carbohydrate-binding characteristics of antibodies, proteins, bacteria, and cell cultures. We also produce custom glycan arrays on demand from our large library of glycans.
Brief History
Glycan microarrays, a set of immobilized glycans printed on multi-well plates, were introduced in 2002, naturally following the advent of successful DNA and recombinant protein microarrays during the late 20th century. With glycan array technology, analysis of glycan binding proteins prevalent in mammals, pathogens, and immunity has aided research for cancer biomarkers, vaccine production, and medical diagnostics. *1
- Identify glycan binding partners in biological samples
- Identify whether target proteins are carbohydrate-binding
- Probe binding of viruses and whole cells to glycans *2-4
- Profile the substrate specificity of enzymes *5-7
- Profile the inflammatory immune response
How It Works
- Immobilization – Printed glycans are non-covalently immobilized to individual wells utilizing close-structure reducing ends of glycans, typically ketones or aldehydes, and chemistry. For example, hydrazide functionalized slides utilize Shapiro’s reaction to immobilize glycans.
- Protein Binding – A naturally isolated or chemo-enzymatically synthesized glycan-binding protein is applied to the slide.
- Fluoresence Labeling – Fluorescent label is directly applied to biotinylated proteins or attached to a secondary antibody.
- Detection – The array is screened using a fluorescence detector.
- Products
- Display 6 Products per page
- References
- 1. Rillahan, C. D. & Paulson J. C. Annu Rev Biochem, 2011, 80, 797-823.
2. Amonsen, M.; Smith, D. F.; Cummings, R. D.; Air, G. M. J Virol 2007, 81, 8341-8345.
3. Paulson, J. C.; de Vries, R. P. Virus Res 2013, 178, 99-113.
4. Stencel-Baerenwald, J. E.; Reiss, K.; Reiter, D. M.; Stehle, T.; Dermody, T. S. Nat Rev Microbiol 2014, 12, 739-749.
5. Park, S.; Shin, I. Org Lett 2007, 9, 1675-1678.
6. Pourceau, G.; Chevolot, Y.; Goudot, A.; Giroux, F.; Meyer, A.; Moules, V.; Lina, B.; Cecioni, S.; Vidal, S.; Yu, H.; Chen, X.; Ferraris, O.; Praly, J. P.; Souteyrand, E.; Vasseur, J. J.; Morvan, F. Chembiochem 2011, 12, 2071-2080.
7. Laurent, N.; Voglmeir, J.; Flitsch, S. L. Chem Commun 2008, 4400-4412.
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