α-2,3-sialyltransferase; PmST1

E. coli recombinant α-2,3-sialyltransferase from Pasteurella multocida (P-1059)

E.C. No.:      2.4.99.4
CAzy:           GT80
MW:             46 kDa
Purity:           min 95% by SDS-PAGE

Unit Define:
One unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol Siaα2,3Lac from CMP-Sia and Lactose per minute at 37 °C.

Reaction catalysed:
CMP-N-acetylneuraminate beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-R <=> CMP alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-R

• The acceptor is Gal-beta-1,3-GalNAc-R, where R is H, a threonine or serine residue in a glycoprotein, or a glycolipid
• Lactose can also act as acceptor

References:
JACS, 2005, 127, 17618-17619.